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KMID : 0545120100200040727
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 4 p.727 ~ p.731
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An efficient method for the expression and reconstitution of thermostable Mn/Fe superoxide dismutase from Aeropyrum pernix K1
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Lee Hee-Jin
Kwon Hye-Won
Koh Jong-Uk
Lee Dong-Kuk
Moon Ja-Young
Kong Kwang-Hoon
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Abstract
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The gene APE0743 encoding the superoxide dismutase (ApSOD) of a hyperthermophilic archaeon Aeropyrum pernix K1 was cloned and over-expressed as a GST fusion protein at a high level in Escherichia coli. The expressed protein was simply purified by the process of glutathione affinity chromatography and thrombin treatment. The ApSOD was a homodimer of 25 kDa subunits and a cambialistic SOD which was active with either Fe(II) or Mn(II) as a cofactor. The ApSOD was highly stable against high temperature. This thermostable ApSOD is expected to be applicable as a useful biocatalyst for medicine and bio-industrial processes.
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KEYWORD
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Aeropyrum pernix, APE0743, Superoxide dismutase, GST-tag, Reconstitution, Thermostable enzyme
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